| Date | 2 September 1960 — 16:00 |
|---|---|
| Location | Stadio Olimpico, Roma |
| Pos | Lane | Competitor | NOC | Time (Hand) | Time (Automatic) | |||
|---|---|---|---|---|---|---|---|---|
| 1 | 6 | Glenn Davis |  USA | 49.3 | 49.51 | |||
| 2 | 5 | Cliff Cushman | USA | 49.6 | 49.77 | |||
| 3 | 1 | Dick Howard | USA | 49.7 | 49.90 | |||
| 4 | 2 | Helmut Janz |  GER | 49.9 | 50.05 | |||
| 5 | 4 | Jussi Rintamäki |  FIN | 50.8 | 50.98 | |||
| 6 | 3 | Bruno Galliker |  SUI | 51.0 | 51.11 |
