| Date | 25 September 1988 |
|---|---|
| Location | Su-yeong-jang, Ol-lim-pik Gong-won, Seoul |
| Pos | Lane | Competitor | NOC | Time | |||
|---|---|---|---|---|---|---|---|
| 1 | 6 | Diane van der Plaats |  NED | 26.49 | |||
| 2 | 5 | Adriana Pereira |  BRA | 26.56 | |||
| 3 | 4 | María Rivera |  MEX | 27.16 | |||
| 4 | 2 | Annabelle Cripps |  GBR | 27.17 | |||
| 5 | 1 | Karen Dieffenthaler |  TTO | 27.27 | |||
| 6 | 8 | Akiko Thomson |  PHI | 27.43 | |||
| 7 | 3 | Patricia Kohlmann | MEX | 27.45 | |||
| 8 | 7 | Elsa Freire |  ANG | 29.54 |
