| Date | 6 September 1960 — 15:45 |
|---|---|
| Location | Stadio Olimpico, Roma |
| Pos | Lane | Competitor | NOC | Time (Hand) | Time (Automatic) | |||
|---|---|---|---|---|---|---|---|---|
| 1 | 3 | Otis Davis | 44.9 | 45.07 | ||||
| 2 | 1 | Carl Kaufmann | 44.9 | 45.08 | ||||
| 3 | 4 | Malcolm Spence | 45.5 | 45.60 | ||||
| 4 | 5 | Milkha Singh | 45.6 | 45.73 | ||||
| 5 | 6 | Manfred Kinder | 45.9 | 46.04 | ||||
| 6 | 2 | Earl Young | 45.9 | 46.07 |