| Date | 6 September 1960 — 15:45 |
|---|---|
| Location | Stadio Olimpico, Roma |
| Pos | Lane | Competitor | NOC | Time (Hand) | Time (Automatic) | |||
|---|---|---|---|---|---|---|---|---|
| 1 | 3 | Otis Davis |  USA | 44.9 | 45.07 | |||
| 2 | 1 | Carl Kaufmann |  GER | 44.9 | 45.08 | |||
| 3 | 4 | Malcolm Spence |  RSA | 45.5 | 45.60 | |||
| 4 | 5 | Milkha Singh |  IND | 45.6 | 45.73 | |||
| 5 | 6 | Manfred Kinder | GER | 45.9 | 46.04 | |||
| 6 | 2 | Earl Young | USA | 45.9 | 46.07 |
