| Date | 3 September 1960 |
|---|---|
| Location | Stadio Olimpico, Roma |
| Pos | Number | Competitor | NOC | Time (Hand) | Time (Automatic) | |||
|---|---|---|---|---|---|---|---|---|
| 1 | 400 | Otis Davis |  USA | 45.9 | 46.02 | |||
| 2 | 109 | Malcolm Spence |  RSA | 46.1 | 46.21 | |||
| 3 | 49 | Abdul Karim Amu |  NGR | 46.6 | 46.76 | |||
| 4 | 519 | Jerzy Kowalski |  POL | 46.7 | 46.82 | |||
| 5 | 599 | Giuseppe Bommarito |  ITA | 47.5 | 47.71 | |||
| 6 | 82 | Barry Robinson |  NZL | 48.3 | 48.44 | |||
| 7 | 235 | Louis De Clerck |  BEL | 48.4 | 48.51 |
