| Date | 26 July 1976 |
|---|---|
| Location | Stade olympique, Parc olympique, Montréal, Québec |
| Pos | Lane | Competitor | NOC | Time (Automatic) | |||
|---|---|---|---|---|---|---|---|
| 1 | 60 | Rick Mitchell |  AUS | 45.76 | |||
| 2 | 217 | Alberto Juantorena |  CUB | 45.92 | |||
| 3 | 729 | Jerzy Pietrzyk |  POL | 46.30 | |||
| 4 | 170 | Bryan Saunders |  CAN | 46.42 | |||
| 5 | 573 | Leighton Priestley |  JAM | 46.45 | |||
| 6 | 76 | Mike Sands |  BAH | 46.48 | |||
| 7 | 764 | Iván Mangual |  PUR | 46.56 | |||
| 8 | 848 | Charles Joseph |  TTO | 46.61 |
