| Date | 27 September 2000 |
|---|---|
| Location | Olympic Stadium, Olympic Park, Sydney, New South Wales |
| Pos | Lane | Competitor | NOC | Time | |||
|---|---|---|---|---|---|---|---|
| 1 | 5 | Mário Aníbal |  POR | 48.71 | |||
| 2 | 4 | Zsolt Kürtösi |  HUN | 48.81 | |||
| 3 | 8 | Fedir Laukhin |  UKR | 49.64 | |||
| 4 | 2 | Klaus Ambrosch |  AUT | 50.23 | |||
| 5 | 7 | Laurent Hernu |  FRA | 50.63 | |||
| 6 | 3 | Prodromos Korkizoglou |  GRE | 53.57 |
