| Date | 18 August 2004 — 20:20 |
|---|---|
| Location | Olympiako Kentro Ugrou Stivou, Olympiako Athletiko Kentro Athinon Spiros Loues, Athina (Main Pool) |
| Pos | Lane | Competitor | NOC | Time | |||
|---|---|---|---|---|---|---|---|
| 1 | 5 | Pieter van den Hoogenband |  NED | 48.17 | |||
| 2 | 4 | Roland Schoeman |  RSA | 48.23 | |||
| 3 | 8 | Ian Thorpe |  AUS | 48.56 | |||
| 4 | 1 | Ryk Neethling | RSA | 48.63 | |||
| 5 | 3 | Filippo Magnini |  ITA | 48.99 | |||
| 6 | 7 | Duje Draganja |  CRO | 49.23 | |||
| =7 | 2 | Salim Iles |  ALG | 49.30 | |||
| =7 | 6 | Andrey Kapralov |  RUS | 49.30 |
