| Date | 12 August 2008 — 18:53 |
|---|---|
| Location | Beijing Guojia Youyong Zhongxin, Beijing Aolinpike Gongyuan, Beijing |
| Pos | Lane | Competitor | NOC | Time | |||
|---|---|---|---|---|---|---|---|
| 1 | 4 | Eamon Sullivan |  AUS | 47.80 | |||
| 2 | 5 | Stefan Nystrand |  SWE | 47.83 | |||
| 3 | 6 | Pieter van den Hoogenband |  NED | 47.97 | |||
| 4 | 3 | César Cielo Filho |  BRA | 48.16 | |||
| 5 | 2 | Andrey Grechin |  RUS | 48.50 | |||
| 6 | 8 | Christian Galenda |  ITA | 48.55 | |||
| 7 | 1 | Joel Greenshields |  CAN | 49.04 | |||
| 8 | 7 | Ryk Neethling |  RSA | 49.28 |
