| Date | 5 August 1928 — 14:15 |
|---|---|
| Location | Olympisch Stadion, Amsterdam |
| Pos | Lane | Competitors | NOC | Time (Hand) | |||
|---|---|---|---|---|---|---|---|
| 1 | 5 | United States |  USA | 41.0 | |||
| Lead-Off | 569 | Frank Wykoff | – | ||||
| 2nd Leg | – | Jimmy Quinn | – | ||||
| 3rd Leg | 508 | Charley Borah | – | ||||
| Anchor | 557 | Hank Russell | – | ||||
| 2 | 3 | Germany |  GER | 41.2 | |||
| Lead-Off | 727 | Georg Lammers | – | ||||
| 2nd Leg | 707 | Richard Corts | – | ||||
| 3rd Leg | 717 | Hubert Houben | – | ||||
| Anchor | 722 | Helmut Körnig | – | ||||
| 3 | 2 | Great Britain |  GBR | 41.8 | |||
| Lead-Off | 456 | Cyril Gill | – | ||||
| 2nd Leg | 486 | Teddy Smouha | – | ||||
| 3rd Leg | 480 | Walter Rangeley | – | ||||
| Anchor | 469 | Jack London | – | ||||
| 4 | 4 | France |  FRA | 42.0 | |||
| Lead-Off | 56 | André Cerbonney | – | ||||
| 2nd Leg | 51 | Gilbert Auvergne | – | ||||
| 3rd Leg | 66 | André Dufau | – | ||||
| Anchor | 86 | André Mourlon | – | ||||
| 5 | 6 | Switzerland |  SUI | 42.6 | |||
| Lead-Off | 268 | Emmanuel Goldsmith | – | ||||
| 2nd Leg | 279 | Willy Weibel | – | ||||
| 3rd Leg | 278 | Willy Tschopp | – | ||||
| Anchor | 274 | Hans Niggl | – | ||||
| 1 | Canada |  CAN | – | ||||
| Lead-Off | 645 | Ralph Adams | – | ||||
| 2nd Leg | 651 | Johnny Fitzpatrick | – | ||||
| 3rd Leg | 654 | Buck Hester | – | ||||
| Anchor | 667 | Percy Williams | – |
